100 bp dna ladder promega pdf file

The 500 bp and 1,500 bp bands contain more dna to provide internal orientation. The ladder is prepared from a plasmid containing repeats of a 100 bp dna fragment. Promega 100bp dna step ladder molecular weight marker for electrophoresis 100bp dna step ladder 100 to 4,000. Generuler 100 bp dna ladder thermo fisher scientific. Promega 100bp dna ladder molecular weight marker 11 fragments from 100bp to 1,000bp in 100bp increments and includes an additional 1. For the labeling of the dna, the product is simply dissolved in te buffer or bidistilled water. Dna step ladders have defined sizes with exact incremental steps between bands. They are great when more specific fragment length verification is desired than can be inferred from a typical ladder with larger, or less consistent increments. The 600 bp band is approximately 2 to 3 times brighter. Dna staining with methylene blue occasionally, ethidium bromide is not suitable for staining dna. Laboratory production of 100 base pair dna molecular weight. Dna marker dna ladder 100 bp plus geneonbioscience.

From promega you will see that there is no indication given for the amounts of the individual bands. Dna marker 100 bp plus is designed for use as qualitative dna reference marker availibility of sample size may be limited 306005 50 g 29,00 306025 5x50 g 99,00 free sample s304005 sample size 0,00. It contains bromophenol blue dye which serves as visual aid to monitor the progress of migration during agarose gel electrophoresis 1kb plus dna ladder, 100 10kb, 1 x 500ul vial. The 100 bp dna ladder is a unique combination of a number. Forty bluntended dna fragments ranging from 100bp to 4,000bp in. Three dna markers, low range, high range, and broad range that allow fast sizing of a wide range of dna fragments 125 bp to 10 kbmolecularweight size markers are a set of standards used to identify. The 1kb dna ladder has thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. Tracking dye does not leave a shadow under uv light. The ladder is generated from pcr and restriction enzyme digestion of double stranded dna. All products sold on the site are available regardless of which option you choose. Three dna markers, low range, high range, and broad range that allow fast sizing of a wide range of dna fragments 125 bp to 10 kbmolecularweight size markers are a set of standards used to identify the approximate size of a molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel matrix. The 100 bp dna ladder ranges from 100bp 1517 bp and is supplied with a free vial of gel. The approximate mass of dna in each band is provided 0.

The 100 bp dna ladder is suitable for sizing doublestranded dna fragments from 1002000 bp. The 100 bp dna ladder is suitable for sizing doublestranded dna from 100 to 1,500 bp. Dna ladders have defined sizes, and are not intended for use in quantitative analysis. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromidestained agarose gels for easy identification. The 100 bp dna ladder is suitable for sizing linear doublestranded dna fragments from 100 bp to bp. The 100 bp dna ladder contains 11 discrete dna fragments ranging in size from 100 bp to 3,000 bp. Gentledna markers wikibooks, open books for an open world. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Email promega technical services if you have questions on use of this system.

The dna fragments may be stained with ethidium bromide. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Each ladder is provided with a tube of 6x blueorange loading dye. The dna ladder consists of proprietary plasmids which are. A verified email address is required to access the full functionality of your account. Dna marker 100 bp plus no dye the 100 bp marker bp is designed for use as molecular weight standard for agarose gel electrophoresis features. The 100 bp dna ladder consists of 15 bluntended fragments between 100 and 1500 bp in multiples of 100 bp and an additional fragment at 2072 bp. Invitrogen 100 bp dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2,000 bp. The 500 bp band with higher concentration is distinguished easily from others. The 600 bp band is approximately 2 to 3 times brighter than the other ladder bands to provide internal orientation. The ladders are not intended for use in quantitative analysis. Identification of the preparationsubstance and of the company preparation name.

Invitrogen trackit 100 bp dna ladder is ready to use and designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2,000 bp. Thirteen bluntended fragments with sizes ranging from 250bp to 10,000bp. The concentration of 1kb dna ladder is 100 ugml 500ul five microlitres 500 ng of the 1 kb dna ladder are mixed with 1ul of blueorange 6x loading dye. The ladder is composed of chromatographypurified dna fragments dissolved in te buffer 10 mm. Quickload purple 100 bp dna ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Quickload purple 1 kb dna ladder is stable for at least 6 months at 25c. The readytouse 100bp ladder, premixed with loading buffer dye, ranges from 1,500 to 100 bp and has intense reference bands at 1500bp and 500 bp. From promegayou will see that there is no indication given for the amounts of the individual bands. A highintensity 500bp band stands out at the lowest segment of the ladder 1kb. The 100bp dna ladder has eleven fragments that range in size the 500bp fragment is present at.

Quick load 100 bp dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. Thirteen bluntended fragments with sizes ranging from. Two readytouse dna size markers for gel electrophoresis, the 100 bp ladder and 1 kb ladder represent the two most poplar markers used for dna fragment size determination. A highintensity 500bp band stands out at the lowest segment of the ladder ladder is dephosphorylated and ready for 5 endlabeling with radioisotopes using t4 polynucleotide kinase, allowing visualization by autoradiography. The 100 bp dna ladder is formulated to run accurately and to provide crisp band patterns. Product components and storage conditions smallscale isolation minipreps product size cat. Invitrogen 100 bp dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2000 bp.

We have estimated the amounts of the individual bands to be as follows for 6 microliters per lane. The 100bp dna step ladder has 40 bluntended dna fragments ranging from 100bp to 4,000bp in 100bp increments. Page formattingtable formatting, incorrectmissing document pdf, other. Promega 100bp dna ladder molecular weight marker 100 bp dna. The 25bp dna step ladder has 12 dna fragments ranging from 25bp to 300bp in 25bp increments. Forty bluntended dna fragments ranging from 100bp to 4,000bp in 100bp increments. If samples need to be diluted, use te or other buffer of minimal ionic strength. The 500bp fragment is present at increased intensity for easy identification. The 100bp dna ladder has eleven fragments that range in size from 100bp to 1,000bp in 100bp increments with an additional band at 1,500bp. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended fragments ranging in length from 100 to 1,500 bp, at 100. The digested dna includes fragments ranging from 100 bp to 1,517 bp. Promega 100bp dna step ladder molecular weight marker for electrophoresis forty bluntended dna fragments ranging from 100bp to 4,000bp in 100bp increments. Pdf straightforward procedure for laboratory production of dna.

D1035 1 x 500 double strand dna from 100 to 10,200 base pairs. The dna includes fragments ranging from 1003,000 base pairs. For example, step ladder dna fragments could be in increments of 100bp from 100bp all the way up to 4000bp. The 100 bp dna ladder is suitable for sizing doublestranded dna fragments from 100 2000 bp. Agarose gel analysis shows that the bands between 100 bp and 1,500 bp are accurate in size and distinguishable. This marker is ideal for the size determination of pcr products. Promega 100bp dna step ladder molecular weight marker for. Straightforward procedure for laboratory production of dna ladder. Prepared from a plasmid containing repeats of a 100 bp dna fragment, the ladder consists of 15 bluntended.

Laboratory production of 100 base pair dna molecular. Eleven fragments that range in size from 100bp to 1,000bp in 100bp increments plus a band at 1,500bp. Generuler 100 bp dna ladder 250 5 x 50 g for 500 applications, 0. The quickload purple 1 kb dna ladder is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. All bands except the 800bp band are of equal intensity. The dna includes fragments ranging from 1001,500 base pairs. Our teams are in regular contact with suppliers and distributors worldwide and are taking all. Agarose gel analysis must show that bands between 100 1500 bp are distinguishable after ethidium bromide staining. How can i prepare working solution with 1 kb dna ladder.

The 100 bp dna ladder ranges from 100bp 1517 bp and is supplied with a free vial of gel loading dye, purple 6x, no sds. The pcr markers may be run on polyacrylamide gels with less loading volume. The 10bp dna step ladder has ten bluntended dna fragments ranging from 10bp to 100bp in exactly 10bp increments. Promega 100bp dna ladder molecular weight marker 100 bp. The 500 bp band with higher concentration is distinguished easily from. Promega 100bp dna ladder molecular weight marker 100 bp dna ladder. Step ladders are not intended for concentration estimations. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. Trackit 100 bp dna ladder thermo fisher scientific. The loading buffer provided contains density agents and two blue electrophoresis tracking dyes that run at approximately 1. Pdf dna ladder is commonly used to determine the size of dna fragments by electrophoresis in routine molecular. Supplied with free vial of gel loading dye, purple 6x.